| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Malkowicz, Sophia C (10)
|
MRTRI |
42.85 |
42.00 |
-0.85 |
| 2 |
Jukic, Emily N (10)
|
MRVIP |
44.29 |
42.85 |
-1.44 |
| 3 |
Bak, Julia (10)
|
MRQNS |
44.70 |
43.62 |
-1.08 |
| 4 |
Decasseres, Brianna O (10)
|
MRWEST |
44.22 |
44.06 |
-0.16 |
| 5 |
Birch, Elizabeth K (10)
|
MRAGUA |
46.03 |
44.24 |
-1.79 |
| 6 |
Cinek, Alexandra (10)
|
MRPAC |
39.61 |
44.56 |
4.95 |
| 7 |
Doran, Shaelee A (10)
|
MRIA |
43.69 |
44.60 |
0.91 |
| 8 |
Messinger-Nanni, Greta (9)
|
MRAGUA |
48.57 |
44.76 |
-3.81 |
| 9 |
Galimi, Alexa A (10)
|
MRBAD |
44.56 |
44.83 |
0.27 |
| 10 |
Curinnaia, Elena (10)
|
MRMPNY |
43.74 |
45.11 |
1.37 |
| 11 |
Bendersky, Hailey R (10)
|
MRWISC |
45.09 |
46.53 |
1.44 |
| 12 |
Green, Daphne B (9)
|
MRRA |
46.81 |
46.84 |
0.03 |
| 13 |
Shlivko, Yuri (9)
|
MRMPNY |
46.08 |
46.86 |
0.78 |
| 14 |
Suharev, Anna M (10)
|
MRFREE |
46.09 |
46.99 |
0.90 |
| 15 |
Vitiello, Alexis L (10)
|
MRWISC |
46.40 |
47.02 |
0.62 |
| 16 |
Spencer-Edwards, Lauren A (10)
|
MRYMID |
47.81 |
47.10 |
-0.71 |
| 17 |
Yang, Chloe (10)
|
MRAGUA |
48.12 |
47.17 |
-0.95 |
| 18 |
Tu, Sophie G (10)
|
MRYFD |
48.95 |
47.27 |
-1.68 |
| 19 |
Zhang, Kaitlyn H (9)
|
MRPAC |
48.82 |
47.38 |
-1.44 |
| 20 |
Wang, Emily (9)
|
MRLIAC |
49.44 |
47.52 |
-1.92 |
| 21 |
Baynon, Lily M (10)
|
MRFA |
48.53 |
47.69 |
-0.84 |
| 22 |
Kitlowski, Kara O (9)
|
MRLIAC |
48.68 |
47.91 |
-0.77 |
| 23 |
Barron, Emma H (10)
|
MRWISC |
49.01 |
47.94 |
-1.07 |
| 24 |
Meliere, Lily M (10)
|
MRWISC |
46.98 |
48.22 |
1.24 |
| 25 |
Park, Esther Y (10)
|
MRTS |
49.37 |
49.09 |
-0.28 |
| 26 |
Johnson, Mayrah V (9)
|
MRAGUA |
49.44 |
51.07 |
1.63 |
| 26 |
McMahon, Reilly E (10)
|
MRWISC |
49.14 |
51.07 |
1.93 |
| 28 |
Lin, Rong Jia L (10)
|
MRQNS |
49.45 |
51.26 |
1.81 |